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Lymphocytes - A Practical Approach

45,04€ + 10% CARD

 Cellular immunology is a rapidly moving field in which recent advances have made significant contributions to our understanding of the immune response to infection and malignancy. These in turn, have given rise to new therapeutic opportunities in areas such as vaccines and immunotheraphy. Many investigators have been discourages by the complicated protocols involved in cellular immunological studies, as illustrated, by the meticulous care required for the generation of antigen-specific T-cells. Lymphocytes: A Practical Approach (second edition) contains straight-forward protocols for well- established procedures in the study of lymphocytes including preparation and identification of lymphocytes, immortalization, cell and organ culture, and quantification assays. It also covers the recent technological advances which have revolutionised the field, such as the use of the Interferon-gamma ELISpot assay and peptide-HLA tetrameric assays to quantify antigen-specifidc T-cells directly from peripheral blood, without the need for in vitro culture, and molecular methods for accurate HLA typing.
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Fmoc Solid Phase Peptide Synthesis - A Practical Approach

55,64€ + 10% CARD

 In the years since the publication of Atherton and Sheppard's volume, the technique of Fmoc solid-phase peptide synthesis has matured considerably and is now the standard approach for the routine production of peptides. The basic problems at the time of publication of this earlier work have now for the most part, been solved. As a result, innovators in the field have focussed their efforts to develop methodologies and chemistry for the synthesis of more complex structures. The focus of this new volume is much broader, and covers the essential procedures for the production of linear peptides and more advanced techniques for preparing cyclic, side-chain modified, phospho- and glycopeptides. Many other methods also deserving attention have been included: convergent peptide synthesis; peptide-protein conjugation; chemoselective ligation; and chemoselective purification. The difficult preparation of cysteine and methionine-containing peptides is also covered, as well as methods for overcoming aggregation during peptide chain assembly. Many of the techniques developed for the production of large arrays of peptides by parallel synthesis, such as t-bag, SPOT and PIN synthesis, have naturally been included. Finally, a survey of available automated instrumentation has also been provided.
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Protein Expression - A Practical Approach

37,09€ + 10% CARD

 Protein Expression: A Practical Approach and its companion volume Post-translational Modification: A Practical Approach complete the mini-series of Practical Approach books covering the synthesis and subsequent processing of proteins. Protein Expression: A Practical Approach details the expression of cloned DNA or RNA templates in all the major in vivo and in vitro systems. The in vivo systems covered are cultured mammalian cells, the yeasts Saccharomyces cerevisiae and Pichia pastoris, baculovirus, Xenopus oocytes, and prokaryotic cells. Cell-free systems of both eukarytotes and prokaryotes are described, including the prokaryotic systems that offer copuled transcription- translation. There is also a chapter on monitoring protein expression. The post- translational fate of proteins is covered in Post-Translational Processing: A Practical Approach.
ENGLISH

Protein Purification Applications - A Practical Approach

62,26€ + 10% CARD

 Proteins are an integral part of molecular and cellular structure and function and are probably the most purified type of biological molecule. In order to elucidate the structure and function of any protein it is first necessary to purify it. Protein purification techniques have evolved over the past ten years with improvements in equipment control, automation, and separation materials, and the introduction of new techniques such as affinity membranes and expanded beds. These developments have reduced the workload involved in protein purification, but there is still a need to consider how unit operations linked together to form a purification strategy, which can be scaled up if necessary. The two Practical Approach books on protein purification have therefore been thoroughly updated and rewritten where necessary. The core of both books is the provision of detailed practical guidelines aimed particularly at laboratory scale purification. Information on scale-up considerations is given where appropriate. The books are not comprehensive but do cover the major laboratory techniques and common sources of protein. Protein Purification Techniques focuses on unit operations and analytical techniques. It starts with an overview of purification strategy and then covers initial extraction and clarification techniques. The rest of the book concentrates on different purification methods with the emphasis being on chromatography. The final chapter considers general scale-up considerations. Protein Purification Applications describes purification strategies from common sources: mammalian cell culture, microbial cell culture, milk, animal tissue, and plant tissue. It also includes chapters on purification of inclusion bodies, fusion proteins, and purification for crystallography. A purification strategy that can produce a highly pure single protein from a crude mixture of proteins, carbohydrates, lipids, and cell debris to is a work of art to be admired. These books (available individually or as a set)are designed to give the laboratory worker the information needed to undertake the challenge of designing such a strategy.
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Cytokine Molecular Biology - A Practical Approach

54,31€ + 10% CARD

 Cytokine Molecular Biology concentrates on molecular biology techniques for the study of cytokines, cytokine receptors, and cytokine driven processes. Updated topics from the previous edition are: the cloning and expressing cytokine genes;; the detection of cytokine mRNA; receptor binding studies; the PC-specific phosphslipase C and sphingomylinases. In addition, new topics covered are the purification, sequencing, and synthesis of cytokines; studying cytokine gene polymorphisms; the use of proteomics in cytokine research; and the Jak/STAT and MAPK signalling pathways. Written by experts in the field, Cytokine Molecular Biology and Cytokine Cellular Biology form a comprehensive and essential guide to cytokine research.
ENGLISH

Apoptosis - A Practical Approach

54,31€ + 10% CARD

 This text is designed to provide conceptual outlines and detailed procedures for basic and advanced studies of cell death by apoptosis. Chapters on the recognition of apoptosis as distinguished from necrosis and nonspecific cell DNA damage, are followed by a systematic examination of the established and the principal novel methodologies utilized by some leading laboratories conducting research on apoptosis. The organization is on the lines of signalling for apoptosis, the apoptotic cascade, and the execution of apoptosis. A wide variety of procedures are provided which will enable the reader to participate in cutting-edge research.
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Cell Growth, Differentiation And Senescence - A Practical Approach

54,31€ + 10% CARD

 There are three main themes running through this volume. First, basic methods for measurement of cell proliferation are introduced and explained with reference to various systems, primarily in vitro, but in vivo procedures are also illustrated. The second theme is growth signalling, and is exemplified by methods for the analysis of transduction pathways for growth, beginning at the cell membrane and leading to the cell nucleus. The last theme presented here is growth cessation, illustrated by several systems for induction of cell differentiation, and of cell senescence. The emphasis throughout the book is on human cell systems, making it particularly relevant to scientists interested in human disease, especially cancer. Importantly, well proved methods for studying cell growth are supplemented by some novel approaches, e.g., studies of cell cycle checkpoints, cell spheroids, and nuclear architecture. Only two chapters have been retained, in an updated form from Cell Growth and Apoptosis, the predecessor volume. The book is written by a team of scientists highly experienced in procedures they describe, and offer details and hints found valuable in their own laboratories; thus, variants of the same general methods can be found in different chapters. These should be helpful to beginning as well as experienced investigators, and are designed to stimulate new approaches to old and new questions.
ENGLISH

Differential Display - A Practical Approach

80,81€ + 10% CARD

 One of the most challenging tasks facing the modern biological research laboratory is to make sense of the enormous amount of data being generated by various genome projects currently underway, and especially the human genome project. Understanding the ways in which genes are differentially expressed in various tissues and cell types, throughout ontogenetic development and in pathological processes, will go a long way towards understanding the function of all these 'new' genes and their protein products. Differential Display explains in detail how to perform the technique of RT-PCR Differential Display in various kinds of experimental biological systems. It also examines this technique in the context of other methods of studying differential gene expression such as subtractive hybridisation and the use of high-density gene microarrays combined with hybridisation techniques and automatic image analysis.
ENGLISH

Combinatorial Chemistry - A Practical Approach

80,81€ + 10% CARD

 Combinatorial Chemistry is a genuine practical guide covering all the major areas of combinatorial chemistry from an experimental and conceptual point of view. Being one of the most powerful of modern technologies, combinatorial chemistry has had implications to many areas of chemistry and biology and the current approaches to drug, catalyst, receptor, and materials development and discovery are all included in this volume. It also contains protocols on solid, liquid, and solution phase synthesis and expedient methods of library screening and evaluation. The use of automation and robotics is also explained. It is written at a level easily accessible to novices and will enable readers to use combinatorial techniques to the best advantage.
ENGLISH

Post-Translational Processing - A Practical Approach

50,34€ + 10% CARD

 Post-translational Modification: A Practical Approach and its companion volume Protein Expression: A Practical Approach form the final part of the PAS mini-series on protein synthesis and processing. This volume begins with a chapter on protein sequencing followed by a chapter on protein folding and import into organelles. The next three chapters cover the three major forms of covalent modification: phosphorylation, glycosylation, and lipid modification. Proteolytic processing the is the next topic and the final two chapters are concerned with protein turnover in mammalian cells and yeast. This book is a comprehensive volume of the best current methodology and is designed to be used at the bench or away from the bench to gain insight into future experimental approaches.
ENGLISH

Nuclear Receptors - A Practical Approach

54,31€ + 10% CARD

 The steroid / nuclear receptor superfamily is a large and growing group of transcription factors that are studied by a large and varied number of basic and clinical researchers. The first two chapters describe the evolutionary biology of the superfamily and explain how to clone and characterize new receptors. Chapter 3 shows how to identify the ligands of novel receptors and chapter 4 explains the kinetic analysis of receptor interactions. In chapter 5, the reader is guided through the functional characterization of coactivators using microinjection. The next section covers receptor phosphorylation, ligand regulated transcription, and hormone resistance syndromes. Chapter 9 describes the in vitro assembly of Hsp90 complexes and chapter 10 explains yeast as a model system for looking at receptor function. The final chapter shows how heterologous proteins can be regulated by fusion to the hormone binding domain of a receptor. Nuclear Receptors: A Practical Approach is a comprehensive guide to studying members of the superfamily and will be invaluable to all researchers old and new.
ENGLISH

Protein Phosphorylation - A Practical Approach

92,74€ + 10% CARD

 Reversible phosphorylation is one of the major mechanisms of controlling protein activity in all eukaryotic cells. This new edition of Protein Phosphorylation: A Practical Approach provides a comprehensive description of current methods used to study protein phosphorylation and the kinases and phosphatases which catalyse it. It includes protocols for studying phosphorylation in intact cells; analysis of signal transduction pathways, kinase specificity, and kinase interactions; assay and purification of kinases and phosphatases; and identification of substrates. Also covered are cloning and expression protocols and advice on the crystallization of kinases and phosphatases. Protein Phosphorylation: A Practical Approach 2e will therefore be of great value to any researcher investigating aspects of reversible protein phosphrylation.
ENGLISH

Signal Transduction - A Practical Approach

54,31€ + 10% CARD

 Since the publication of the first edition of Signal Transduction: A Practical Approach in 1992 there has been a great deal of new information about the processes of signal transduction and consequently many new methods have been developed. This new edition has therefore been updated and extended to include the major new methods now available. The first part of the book is mainly concerned with G protein-coupled receptors and covers structural studies of conformational changes and binding sites, phosphorylation and desensitisation, identification, receptor fusion proteins, and reporter gene systems. The second part includes methods for studying components of the other major families of signal transduction: adenylyl cylase and cAMP, phosphorylated inositol lipids, phosphinositide 3-kinases, phosphlipase D and phosphatidylcholine, sphingosine kinase, and inositol 1,4,5-triphosphate. Also included are chapters on baculoviral expression systems and the quantitative assay of mitogen activated protein kinases in intact cells and tissues. As with the previous edition Signal Transduction 2e covers a wide range of techniques and will be useful to both experienced researchers and newcomers.
ENGLISH

Dna Viruses - A Practical Approach

80,81€ + 10% CARD

 DNA Viruses: A Practical Approach groups together the major experimental methods currently employed to study DNA viruses, from the fundamentals of virus culture to novel techniques such as surface plasmon resonance spectrometry and realtime PCR analysis of drug resistance mutations in clinical isolates. Chapter 1 provides an overview of the extraction, purification and characterizations of virus DNA, but also covers the fundamentals of DNA virus culture. Chapters 2 and 3 describe approaches to the molecular investigation and mutagenesis of DNA virus genomes. Chapter 4 considers DNA virus replication and then chapters 5 & 6 describe methods to study transcription control. Chapters 7 to 9 consider aspects of the pathogenesis of DNA virus infections. The final chapter describes the current technology being applied to the development of DNA virus vectors for gene delivery. This volume will therefore be of interest to all those working on DNA viruses whether in academia, industry or clinical research.
ENGLISH

Virus Culture - A Practical Approach

54,31€ + 10% CARD

 Virus Culture: A Practical Approach provides a broad treatment of the principles and practice of virus culture and will be of interest to all those, whether in academic, industrial, or clinical research, involved in virus culture. The first chapter is an overview of cell culture techniques essential for virologists. Other techniques then covered are isolating, identifying, concentrating, and purifying viruses. Electron Microscopy as applied to virology is also explained. Chapter 6 is about creating virus vaccines and chapters 7 and 8 cover antiserum production, monoclonal antibodies and antiviral drug testing. The final chapter describes the methods used to study plant viruses.
ENGLISH

Mouse Genetics And Transgenics - A Practical Approach

62,26€ + 10% CARD

 A unique book that integrates knowledge from a wide range of expertise, specifically applied to the mouse, and addressed at a wide audience from those new to the field to experts who want an update on the state of the art. Mouse Genetics and Transgenics covers all aspects of using the mouse as a genetic model organism: care & husbandry; archiving stocks as frozen embryos or sperm; making new mutations by chemical mutagenesis; transgenesis; and gene targetting; mapping mutations and polygenic traits by cytogenetic, genetic, and physical means; and disseminating and researching information via the Internet.
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Membrane Transport - A Practical Approach

80,81€ + 10% CARD

 Membrane Transport is targeted towards researchers with an interest in the mechanism of solute transport across biological membranes. Its scope is broad, ranging from the techniques required to study transport itself, through the expression, purification and reconstitution of transporters, to techniques for investigation of their structures. As such, it not only proves the necessary technical grounding for newcomers to the field, but should also be of value to "old-hands" wishing to get up to date with recent developments in these areas. While some of the approaches described require sophisticated equipment (e.g. a stopped-flow fluorimeter), most of the protocols can be implemented in any well- found laboratory. Preparation of this volume comes at a time when a result of genome sequencing our knowledge of membrane transporter sequences is far outstripping our understanding of their molecular mechanisms. Our hope is that this book will help future researchers to redress this imbalance.
ENGLISH

Image Processing And Analysis - A Practical Approach

54,31€ + 10% CARD

 A wide range of books on image processing and analysis provide comprehensive descriptions of mathematics and algorithms for image processing practitioners, or introductory material for engineering students. This volume is different in addressing the topic from the point of view of the "user". Standard algorithms, procedures and rules of thumb are explained in the context of successful application to biological or medical images. Early chapters cover the basic topics of image acquisition, processing, analysis and pattern recognition. Much of the explanation is in the form of protocols, which should equip the user in the biological or earth sciences with the background for informed use of image processing software, and sufficient knowledge to write their own programmes if they feel moved to do so. More advanced techniques in the use of explicit models and analysis of 3D images are covered in later chapters, also with reference to specific applications. The coverage of these is not exhaustive, but may inspire the reader to consider applying image analysis to problems beyond those tackled by commercial packages.
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Chromosome Structural Analysis - A Practical Approach

54,31€ + 10% CARD

 The DNA of eukaryotes is packaged into chromosomes - each chromosome consisting of a very long molecule of DNA and various proteins (e.g. histones), and the number of chromosomes being characteristic for the species concerned. Chromosome analysis can provide a great deal of information for many aspects of cellular genetics such as DNA replication, protein:DNA interactions and genetic manipulation. The book is structured in a methodical fashion - the introductory chapters are centred around analysis of chromatin with chapters on the mapping of protein:DNA interactions in vivo using ligation-mediated PCR and the mapping of chromatin-associated proteins by formaldehyde cross-linking. The next chapters concentrate on the study of whole chromosome structure, including: fission yeast chromosome analysis using FISH and CHIP, isolation of vertebrate metaphase chromosomes and their analysis by FISH, the study of vertebrate chromosome progression through mitosis, and the analysis of mammalian interphase chromosomes by immunofluorescence and FISH. There then follow chapters on FISH in whole-mount tissues and the analysis of the sub-structure of mammalian nuclei in vitro. The final two chapters deal with the experimental manipulation of chromosome structure, including: chromosome assembly in vitro using Xenopus egg extracts and chromosome fragmentation in vertebrate cell lines. This comprehensive and informative laboratory manual includes a diverse range of experimental models for the analysis of chromosomes - such as vertebrates, Drosophila, yeast and Xenopus. Fully illustrated, it focuses on modern techniques and approaches to the study of chromosome structure and will be invaluable to researchers and academic staff in genetics, biomedical science and molecular biology.
ENGLISH

Transcription Factors - A Practical Approach

54,31€ + 10% CARD

 Since the publication of the first edition five years ago, a wide range of new methodologies have been developed to facilitate studies on both isolated parts of the genome and the genome as a whole. This new edition has been updated and expanded so that it provides a comprehensive guide to the methods currently available to characterize the function and activity of an individual transcription factor. All the original chapters have been fully updated or rewritten and additional chapters cover the use of in vitro transcription assays, analysis of chromatin structure, use of the genomic binding site assay and analysis of transcription factor modifications. As with the previous edition, the book starts with a series of chapters concerned with characterizing the proteins binding to a specific DNA sequence and then a chapter on more detailed characterization of the protein itself. The next two chapters describe the isolation of cDNA clones encoding a transcription factor using oligonucleotides predicted from protein sequence and screening of a cDNA expression library. Chapter 6 deals with identification of transcription factors based on sequence homology analysis by both experimental screening and database searches. Chapter 7 is a new chapter that describes methods of identifying the target genes of a previously uncharacterized factor. The next chapters deal with analysis of transcription factor function. Chapter 8 deals with general techniques, and then the following chapters cover the specialized techniques of in vitro transcription assays using transcriptionally active nuclear extracts derived from rat brain, and analysis of the effect of transcription factors on chromatin structure. The final chapter describes methods for detecting the phosphorylation and glycosylation state of transcription factors.
ENGLISH

Eukaryotic Dna Replication - A Practical Approach

54,31€ + 10% CARD

 Eukaryotic DNA Replication: A Practical Approach is a comprehensive practical manual, with each of its eleven chapters describing an aspect of the methods currently used to investigate DNA replication in eukaryotes. The sequence of the chapters corresponds roughly to the order of events during DNA replication. The first chapters are concerned with initiation, looking at methods to characterize origins of replication and the proteins that interact with them. There then follow chapters describing protocols for the study of the elongation phase and the synthesis of the telomeres. The final chapters provide a more general overview of the study of DNA replication - including its investigation in model systems such as yeast, xenopus and viruses, and looks into methods used to study DNA:protein interactions that could be applied to the study of replication proteins. This exciting new volume provides over 120 tried and tested protocols for the analysis of eukaryotic DNA replication and will be of major interest to a wide variety of molecular and cell biologists, biochemists and medical researchers.
ENGLISH

Proteolytic Enzymes - A Practical Approach

62,26€ + 10% CARD

 Like the popular first edition, this new edition of Proteolytic Enzymes emphasizes practical aspects of the handling, characterization, inhibition, and use of proteolytic enzymes giving general advice and specific examples. The text and protocols have been thoroughly updated to take account of the advances made in the last 10 years in both the increased understanding of the role of peptidases in many critical cellular processes e.g. apoptosis and new technological developments e.g. in recombinant protein expression, protein sequencing, and structural studies. The topics covered are: nomenclature and classification; purification; assay methods; determination of mechanism; inhibition and prevention of unwanted proteolytic activity; characterizing natural inhibitors; proteolytic enzymes in peptide mapping and primary structure elucidation by mass spectrometry and Edman sequencing; limited proteolysis as a structural probe; synthetic function. This book will be as invaluable as the first edition in providing ideas and protocols for scientists either studying proteases or using proteases as a research tool.
ENGLISH

High Resolution Chromatography - A Practical Approach

54,31€ + 10% CARD

 The molecular biology revolution has required the development of new chromatographic techniques and the optimization of original techniques to give reasonable quantities of protein at high resolutions. The aim of this volume is to provide the necessary information in most experimental situations to enable rapid and effective purification. The first four chapters deal with the instrumental aspects of high resolution chromatography starting with the initial clean up steps prior to separation in chapter 1. Chapter 2 deals with microscale techniques, then chapter 3 describes the detector technologies that can determine information about the separated molecules. The final chapter in this section cover capillary electrophoresis and its associated techniques. The remaining chapters cover a range of chromatographic procedures based on the interaction of a specific ligand with its target protein or other macromolecule. Some chapters cover non-specific interactions using peptides, inhibitors, and antibodies as the affinity ligand while others focus on specific groups of molecules : oligosaccharides and glycosylated proteins, nucleotide-binding proteins, proteins binding free and chelated metal ions, and DNA binding proteins.
ENGLISH

Growth Factors And Receptors - A Practical Approach

54,31€ + 10% CARD

 Growth Factors and Receptors: A Practical Approach provides comprehensive protocols for studies of growth factors and their interactions with receptors. It covers a wide range from simple analytical techniques to sophisticated in vivo applications including: RT-PCR and immunocytochemistry for detection of growth factors and receptors; production and purification of recombinant growth factors and receptors; labelling of growth factors for binding studies; in vivo mutagenesis; the yeast two-hybrid assay of proteinprotein interactions; phage display of factors; application of factors to wound-healing processes using the gene gun; treatment of cancers with factor/toxin chimeras; and analysis of important factor domains using chimeric proteins. This book updates and extends the current literature and describes important novel approaches to the study of growth factors and their receptors, including the use of RNA aptamers as receptor antagonists, and the development of receptor superantagonists. It will be of tremendous value to both researchers and teachers, and, through an appendix that lists a large number of growth factors and receptors, will serve as a handy reference text.
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